Carbohydrate AnalysisCARBOSep Columns
Transgenomic manufactures a line of polymeric columns for
carbohydrate analysis called CARBOSep columns. CARBOSep
columns employ a technique called ligand-exchange
chromatography for the separation of monosaccharides,
disaccharides and oligosaccharides up to 11 glucose units long.
The principle behind ligand exchange is that each of the hydroxyls
on a sugar molecule carry a very slight negative charge. The
hydroxyl group on the anomeric carbon can be deprotonated and
have a strong negative charge. It is the interaction between these
negative charges on the sugar molecule and the positive charge
contributed by the metal ion secured to the resin surface that
causes the sugars to be retained and thus separated.
Ligand exchange resins are highly sulfonated cation exchange
resins that have group 1, 2 or transition series metals loaded on.
The sulfonic acid groups on the resin tightly hold the metal ions
via an ionic attraction so that it is not released during analysis or
through the life of the column. It is this metal ion that provides the
positive charge that interacts with the negative
charge on the sugar.
During analysis, the carbohydrates are introduced onto the
column. The sugars are attracted to the metals via an ionic
interaction thus they become weakly bound to the metal ion on
the resin. Water will also have a weak ionic interaction with the
metals on the column, so the water will exchange with the sugars
on the metal sites. This ionic adsorption and desorption occurs for
the sugars through the column. Since the ionic charge is different
for every sugar, separation of the sugars occurs.
Selectivity is easily controlled by resin type, metal selected, and
other factors such as temperature and mobile phase. CARBOSep
columns are provided in a large variety of resin types and metals
to provide selectivities that meet your separation needs.
Selectivity Chart for Carbohydrate Columns Carbohydrate Columns Specifications Chart
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